 Pretreatment with phytoestrogen-rich plant decreases breast tumor incidence and exhibits lower profile of mammary ERα and ERβ
Source: Maturitas Volume 58, Issue 2, 20 October 2007, Pages 174-181
Wichai Cherdshewasart, Rattana Panriansaen and Porntipa Picha. Department of Biology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand Program of Biotechnology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok 10330, Thailand
Section of Experimental Oncotherapy, Research Division, National Cancer Institute, Ministry of Public Health, RamaVI Road, Ratchathewi, Bangkok 10400, Thailand
Received 19 May 2007; revised 2 August 2007; accepted 9 August 2007. Available online 17 September 2007.
Abstract
Objective
Phytoestrogens have been reported to exhibit antiproliferation to human breast cancer cells in vitro. We tested the phytoestrogen-rich, Pueraria mirifica against rat breast cancer induction in vivo.
Methods
The weanling female Spargue–Dawley rats were pretreated with P. mirifica tuberous powder at a dosage of 0, 10, 100 and 1000 mg/kg BW/day for four consecutive weeks. Mammary tumor development was then induced with a single dose of 7,12-DMBA, 80 mg/kg BW, followed by a weekly examination for size and multiplicity of mammary tumors for 20 weeks and finally a necropsy. Mammary tissues were investigated for the virulence of tumor and also monoclonal antibody stained against ERα and ERβ.
Results
Pretreatment of 1000 mg/(kg BW day) of Pueraria mirifica tuberous powder resulted in decreasing of the virulence of rat tumor development. The mammary tumor tissues exhibited lower profile of ERα and ERβ as well as ERα/ERβ.
Conclusion
Pueraria mirifica exhibited prevention of 7,12-DMBA-induced rat mammary tumors, with a proposed mechanism of strong competitive binding of its phytoestrogens to ERα and/or synthesis suppressor of ERα.
Keywords: Phytoestrogen ; Breast cancer ; Estrogen receptor ; 7,12-DMBA; Pueraria mirifica ; Isoflavonoid
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